Effect of Different Dentin Conditioning Agents on Release of Growth Factors from Radicular Dentin Discs: A Comparative In-Vitro Study

Document Type : Original Article

Authors

1 Department of Endodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt

2 Department of Pediatric Dentistry and Dental Public Health, Faculty of Dentistry, Cairo University, Cairo, Egypt

10.21608/adjc.2025.330550.1654

Abstract

Aim: To evaluate and compare the effect of dentin conditioners; 10% citric acid, 7% maleic acid, and 17% EDTA on the release of vascular endothelial growth factor (VEGF) and transforming growth factor beta-1 (TGF-β1) from dentin discs.
Subjects and methods: Human dentin discs (n= 15) were disinfected and prepared to obtain a standardized size. The outer diameter is 4-5 mm, while the root canal diameter is 1-2 mm. 17% EDTA, 7% maleic acid (MA), and 10% citric acid (CA) were used as dentin conditioners. After conditioning, dentin discs were placed in PBS (1 mL) and kept at 37˚C for 28 days. An ELISA test was used to quantify VEGF and TGF-β1 in the released medium. ANOVA test was utilized to compare the mean value of growth factors released at a significance level of 0.05. For multiple comparisons between groups, a post hoc Tukey test was used.
Results: It was found that dentin conditioning using CA and MA released significantly more growth factors than EDTA. A statistically significant increase in VEGF and TGF-β1 release was observed in CA group than in MA group.
Conclusion: The release of growth factors by 7% MA and 10% CA was statistically more than 17% EDTA from dentin discs. CA was observed to be significantly more effective than other tested agents in growth factors release.

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