Evaluation of the Effect of Casein Phosphopeptide-amorphous Calcium Phosphate on Fracture Resistance of Single Rooted EDTA Treated Teeth: An In-Vitro Study

Document Type : Original Article

Authors

Endodontics Department , Faculty of dentistry , Cairo university

Abstract

Aim: The aim of this in vitro study was to evaluate the effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on fracture resistance of single rooted ethylenediaminetetraacetic acid (EDTA) treated teeth when utilized as a final irrigant.
Subjects and methods: Twenty-one human teeth with single root and single canals were decoronated at 13+0.5 mm in length. Sodium hypochlorite 5.25 % was used as the standard irrigation regimen during the chemo-mechanical preparation process. In accordance with the final irrigation technique, teeth were randomly assigned to three groups (n=7 in each) as follows: Group (A) received a 1-minute 17% EDTA irrigation followed by a 10-minutes CPP-ACP re-mineralizing solution; Group (B): received a 1- minute 17% EDTA irrigation. Group (C) received a 5-minutes normal saline irrigation. Teeth were mounted in acrylic blocks and then fractured along the tooth's long axis. The fracture resistance was determined by means of a universal testing machine. Data were gathered and subjected to statistical analysis. Significance level was set at p< 0.05.
Results: The results indicated that group A had the highest levels of fracture resistance, followed by group C then group B. Group A's fracture resistance values differed significantly from those of the other groups (p<0.001). The majority of samples in all groups had a vertical fracture and the difference regarding mode of failure distribution was not statistically significant.
Conclusion: After utilizing EDTA to remove the smear layer, the final rinse with the CPP-ACP re-mineralizing solution has significantly increased root dentin fracture resistance.

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