Evaluation of Dentinogenisis of Bionanocomposite Scaffold Versus Mineral Trioxide Aggregate as A Direct Pulp Capping Material for Vital Pulp Therapy: An Animal Study

Hafez, Nada Ashraf; Elzoghbi, Amira Mohamed Farid; Elzayat, Ghada Ahmed; El-Saeed, Marwa A.

doi:10.21608/adjc.2024.303678.1585

Evaluation of Dentinogenisis of Bionanocomposite Scaffold Versus Mineral Trioxide Aggregate as A Direct Pulp Capping Material for Vital Pulp Therapy: An Animal Study

Document Type : Original Article

Authors

¹ Esthetic and Restorative Dentistry, Faculty of Dentistry, Egyptian Russian University, Cairo, Egypt

² Conservative Dentistry Department, Faculty of Dentistry, Cairo University, Cairo, Egypt

³ Conservative Dentistry Department, Faculty of Dentistry, Egyptian Russian University, Cairo, Egypt

⁴ Oral Biology Department, Faculty of Dentistry, Egyptian Russian University, Cairo, Egypt

10.21608/adjc.2024.303678.1585

Abstract

Aim: The aim of this study was to evaluate the ability of bio nanocomposite material (Nano MTA/collagen) to induce dentin bridge formation in comparison to mineral trioxide aggregate material (MTA) when used as direct pulp capping materials. Materials and Method: A total of 32 incisors of rabbits were recruited. Traumatic pinpoint pulp exposure was done in all teeth. Teeth were randomly divided into two groups (n= 16 per group) according to the pulp capping materials. Group (1): innovative bio nanocomposite (NanoMTA/Collagen) and Group (2): conventional mineral trioxide aggregate. Materials were added as direct pulp capping materials and then sealed with conventional glass ionomer restorative material. Histological and Scanning Electron Microscopic examination with EDAX were used to evaluate dentin bride formation and mineral content after one-month time interval. Data were collected, tabulated, and statistically analysed using SPSS 20, Graph Pad Prism and Microsoft Excel 2016. Results: Regarding histological evaluation, no significant difference was found between NanoMTA/Collagen and MTA in inducing dentin bridge formation. Tissue damage and Inflammatory response was higher in MTA compared to NanoMTA/Collagen. Regarding scanning electron microscope with EDAX, MTA group showed highest in calcium and phosphorus weight percentage, while Ca/P ratio was higher in NanoMTA/Collagen group. Conclusions: 1) Nano mineral trioxide aggregate/collagen (NanoMTA/Collagen) and mineral trioxide aggregate (MTA) both induce dentin bridge formation in direct pulp capping therapy. 2) The induced reparative dentin of NanoMTA/Collagen was the closest in morphology and composition to normal dentin. 3) NanoMTA/Collagen was biologically compatible when used as a pulp capping material.

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