Antibacterial efficacy and biocompatibility of Ca (OH)2, camphorated parachlorophenol (CPCP) and a combination of Ca (OH)2 and CPCP as root canal dressing against E. faecalis in single rooted mandibular premolars (A comparative in-vitro study)

Zain Elabdin, Hoda Ibrahim; Negm, Maged Mohamed; Shaker, Olfat Gamil; Morsy, Dina Ahmed

doi:10.21608/adjc.2024.308780.1595

Antibacterial efficacy and biocompatibility of Ca (OH)2, camphorated parachlorophenol (CPCP) and a combination of Ca (OH)2 and CPCP as root canal dressing against E. faecalis in single rooted mandibular premolars (A comparative in-vitro study)

Document Type : Original Article

Authors

¹ Department of Endodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt.

² Endodontist department , faculty of dentistry , cairo university

³ Department of Endodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt

⁴ Department of Endodontics, Faculty of Dentistry, Cairo University.

10.21608/adjc.2024.308780.1595

Abstract

Aim: This in-vitro study aimed to assess and compare the antibacterial efficacy and biocompatibility of Ca(OH)2, camphorated parachlorophenol (CPCP) and a combination of Ca(OH)2 and CPCP as root canal dressings against E. faecalis in single rooted mandibular premolars compared to teeth with no intracanal medications.
Materials and methods: The antimicrobial test: Fifty human extracted single-canalled teeth were collected and prepared.The teeth were sterilized and each root canal was infected with 20 µ of E. faecalis bacteria, which were then randomly categorized into five equal groups based on the intracanal medicament. By counting the number of colonies forming units per milliliter of each dilution on the agar medium, the resulting growth was visually quantified. The biocompatibility test: The BHK normal fibroblast cell line was collected and then cultured (96-well plates for all groups). Three different intracanal medications (Ca(OH)2, CPCP, a combination of Ca(OH)2 and CPCP) were added to the cultured cells. Fresh media were used to culture the cells in the Control Group (CG). Cytotoxicity was evaluated by MTT reduction assay. The significance level was set at P≤ 0.05.
Results: The combination of Ca(OH)2 and CPCP showed the highest anti-bacterial effect followed by intracanal medication with Ca(OH)2. According to the biocompatibility, CPCP group showed the highest cytotoxic effect followed by the combination group and Ca(OH)2 group.
Conclusion: Combining Ca(OH)2 and CPCP shows high antibacterial efficacy compared to each medication alone. Ca(OH)2 was biocompatible on the tissue. Thereafter, comes a mixture of CPCP and Ca(OH)2 then CPCP.

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