Gingival crevicular fluid and salivary levels of neuregulin-4 and its receptor ErbB4 in patients with stage III and IV periodontitis with and without type 2 diabetes: An Observational study

Document Type : Original Article


1 periodontology faculty of oral and dental medicine cairo university

2 Faculty of Dentistry- Cairo University

3 1Department of Oral Medicine and Periodontology, Faculty of Dentistry, Cairo University

4 Department of Biochemistry, Faculty of Medicine, Cairo University


Background: Periodontitis and diabetes mellitus are common diseases that affect a large population worldwide. While researching the possible mechanisms linking periodontitis and diabetes, adipokines emerged as a promising common biological mediator between both diseases. The purpose of this study is to assess the levels of Neuregulin-4, and its receptor ErbB4 in the GCF and saliva of stage III and IV periodontitis patients with and without type II diabetes. Methodology: Saliva and GCF samples were collected from 42 participants, 14 healthy participants, 14 stage III or IV periodontitis patients with diabetes mellitus and 14 non-diabetic stage III or IV periodontitis patients. Samples were analyzed using ELISA and results were correlated with clinical periodontal parameters, glycosylated hemoglobin, and fasting plasma glucose. Results: Elevated levels of Nrg4 and its receptor ErbB4 were found in the periodontitis and diabetes mellitus groups, with a statistically significant difference between the periodontitis and non-diabetic groups, as well as the periodontally healthy group. There was no significant correlation between periodontal clinical parameters, glycosylated hemoglobin, or fasting plasma glucose levels and Nrg4 and its receptor levels. Conclusion: This study demonstrated that the Nrg4-ErbB4 axis plays a role in the pathogenesis of periodontitis and diabetes mellitus.


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