Gingival Crevicular Fluid and Salivary Levels of Galectin-3 in Patient with Gingivitis and Patient with Stage III Periodontitis: An Observational Study

Document Type : Original Article


1 Oral Medicine and Periodontology Department, Faculty of Dentistry, Cairo University, Egypt.

2 Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt.


Aim: The study was aimed at assessing the levels of Galectin-3 in the GCF and Saliva in gingivitis and stage III periodontitis patients. Methodology: The present cross-sectional study was conducted on 66 participants including periodontal healthy, gingivitis, as well as stage III periodontitis individuals. Periodontal examination was continued at the outpatient clinic, Department of Oral Medicine and Periodontology, Faculty of Dentistry, Cairo University. Pocket depth, clinical attachment level, bleeding on probing, and plaque index were measured using a manual UNC periodontal probe. GCF samples were collected from the deepest site by inserting absorbent paper strips into the gingival sulcus for 30 seconds. Five ml of saliva sample was collected from each subject into Eppendorf tubes which were immediately frozen & stored at -70 °C. GCF and Salivary Galectin-3 levels were quantified by using a commercial Enzyme-Linked Immunosorbent Assay Kit. All these parameters were subjected to statistical analysis. Results: There was a statistically significant higher levels of Galectin-3 in the GCF and Saliva of periodontitis than in periodontal healthy. also, gingivitis group had higher levels compared to healthy group, while there was no statistically significant difference between gingivitis and periodontitis. Conclusions: Galectin-3 is a proinflammatory protein that might play essential roles in periodontal disease pathogenesis.