Evaluation of dentinal tubules penetration of bio-ceramic and resin root canal sealers using different obturation techniques: An in-vitro study

Document Type : Original Article


1 Endodontic Department, Faculty of Dentistry, October 6 University, Cairo, Egypt.

2 Biomaterials Department, Faculty of Dentistry, October 6 University, Cairo, Egypt.


Objective: This study was conducted to evaluate contact angle and dentinal tubules penetration depth of Epoxy resin-based sealer Adseal and calcium silicate-based sealer CeraSeal with different obturation techniques using Scanning Electron Microscope (SEM).
Material and Methods: 56 freshly extracted upper central incisors were prepared in the present study. The specimens were divided into four experimental groups (each n= 14) which were filled with different sealers (Ceraseal and Adseal) by different obturation techniques {Cold lateral compaction (CLC) and Warm vertical compaction (WVC)} and respective core filling materials. Group 1 (Ceraseal/ WVC), Group 2 (Ceraseal/CLC), Group 3 (Adseal/ WVC) and Group 4 (Adseal/ CLC). Root slices were cut with a thickness of 1 mm at 2 to 3, 5 to 6 and 8 to 9 mm from the root tip using diamond saw with coolant then scanning electron microscope at 1600x magnification was employed to assess the tubular penetration of different sealers. One Way ANOVA test was used to compare different groups, followed by Tukey`s Post Hoc test for multiple sections comparison.
Results: CeraSeal /CLC group and Adseal / WVC group showed the highest dentinal tubules penetration (11.75±3.72 µm and 10.45±4.73 µm, respectively) with statistically insignificant difference between them (P>0.05). Adseal/CLC group showed the lowest penetration (6.63±2.73 µm), while CeraSeal/ WVC group (9.41±2.71 µm) revealed insignificant difference with the other groups (P>0.05).
Conclusions: Obturation technique should be selected according to sealer type for a successful endodontic treatment. Bioceramic sealer had higher ability of dentinal tubules penetration than epoxy resin sealer.


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