Cairo University, Faculty of Dentistry
Advanced Dental Journal
2636-302X
2636-3038
3
3
2021
07
01
Histomorphometric analysis of bone formation after using simvastatin chitosan nanoparticles as a local delivery system in periodontal bony defects in rabbits
109
119
EN
Wisam
Delan
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Egypt.
ph_wissam@yahoo.com
Basma
Elsaadany
Oral Medicine and Periodontology Department, Faculty of Dentistry, Cairo University, Egypt
basma.elsaadany@yahoo.com
Ahmed
Fares
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Egypt.
ahmed.roshdy@pharma.cu.edu.eg
Aliaa
ElMeshad
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Egypt
aliaa.elmeshad@pharma.cu.edu.eg
Wael
Mamdouh
Department of Chemistry, School of Sciences and Engineering, The American University in Cairo, Egypt.
wael_mamdouh@aucegypt.edu
Mai
Zakaria
0000-0002-2846-6094
Oral Medicine and Periodontology Department, Faculty of Dentistry, Cairo University, Egypt
mai.zakaria@dentistry.cu.edu.eg
10.21608/adjc.2021.74327.1097
<strong>Background:</strong> simvastatin (SV) has a characteristic effect on the regeneration of bony tissues and the decrease of bone resorption. Accordingly, this study aimed to explore the effect of locally applied simvastatin loaded in a chitosan nanoparticles as a delivery system for regeneration of bone in intrabony defects in a rabbit model.<br /> <strong>Materials and methods:</strong> This trial was carried out on 20 male albino New Zealand rabbits. The rabbits were randomly distributed into 4 groups where each group contained 5 rabbits (n=5) as follows: Group 1 (NC group) designed as the negative control, while, interventional groups where an intrabony defect was created designed as: Group 2 (PC) the positive control where the intrabony defects were covered with gingival flaps only. Group 3 (NP) and group 4 (SP) were received chitosan tripolyphosphate nanoparticles (CS-TPP NPs) and medicated SV -CS-TPP NPs respectively. All the animals were euthanized after 6 weeks. Descriptive histological and histomorphometric analysis of specimens stained with hematoxylin-eosin (H & E) and Masson’s trichrome stain (M T) was done.<br /> <strong>Results:</strong> The histomorphometric analysis of area percent of newly bone formation in mm2 showed statistically significant differences between interventional study groups. SV -CS-TPP NPs treated group showed the highest mean of area percent of newly formed bone 37.33 ± 3.82, Group 3 (NP) 18.57 ± 3.42 , while group 2 (PC) showed the lowest one 6.21 ± 2.30.<br /> <strong>Conclusion:</strong> Chitosan nanoparticles as a carrier for local application of simvastatin optimizing the new bone formation in intabony periodontal defects.
Simvastatin,Chitosan,Nanoparticles,Bone Regenerations,Bone Area
https://adjc.journals.ekb.eg/article_184694.html
https://adjc.journals.ekb.eg/article_184694_3c47ff41bcd7399fc5f03d1e1235047f.pdf
Cairo University, Faculty of Dentistry
Advanced Dental Journal
2636-302X
2636-3038
3
3
2021
07
01
SIRT-1 and CD44 in the saliva of oral squamous cell carcinoma, dysplastic and non-dysplastic oral leukoplakia
120
134
EN
Rasha
Wagih
Oral Medicine and Periodontology Department, Faculty of Dentistry, Cairo University
rashawagihmost@gmail.com
Heba
Hussein
0000-0001-6042-361X
Oral Medicine and Periodontology Department, Faculty of Dentistry, Cairo University
heba.said.ismail@gmail.com
Amal
Hussine
Oral Medicine and Periodontology Department, Faculty of Dentistry, Cairo University
amal.hussine@dentistry.cu.edu.eg
10.21608/adjc.2021.70445.1094
<strong>Objective:</strong> The objective of this study was to investigate the levels of salivary SIRT-1 and CD44 in patients suffering from oral squamous cell carcinoma (OSCC), dysplastic and non-dysplastic oral leukoplakia to assess their potential role as early diagnostic biomarkers for patients with oral leukoplakia and OSCC.<br /> <br /><strong>Subjects and Methods:</strong> sixty patients were recruited from the outpatient clinic of the Department of Oral Medicine and Periodontology, Faculty of Dentistry, Cairo University. Participants were divided into four groups:15 healthy individuals, 15 patients with non-dysplastic leukoplakia, 15 patients with dysplastic leukoplakia, and 15 patients with oral squamous cell carcinoma. Whole unstimulated saliva (WUS) was collected from all participants. Soluble CD44 (solCD44) and SIRT1 were measured using ELISA.<br /> <br /> <strong>Results:</strong> CD44 was expressed in all the four study groups with different levels. OSCC showed a statistically significant highest CD44 level. While the control group showed the lowest CD44 level. There was no statistically significant difference between dysplastic leukoplakia and non-dysplastic leukoplakia groups. Similarly, there was no statistically significant difference between non-dysplastic leukoplakia and control groups. OSCC showed the statistically significantly highest SIRT-1 level. There was no statistically significant difference between dysplastic leukoplakia, non-dysplastic leukoplakia, and control groups; all showed statistically significantly lower SIRT-1 levels than the OSCC group.<br /> <br /> <strong>Conclusion:</strong> salivary SIRT-1 and CD44 could be used as a screening tool to improve early detection and diagnostic precision of oral leukoplakia and OSCC.
SIRT-1,CD44,solCD44,OSCC,oral leukoplakia
https://adjc.journals.ekb.eg/article_185222.html
https://adjc.journals.ekb.eg/article_185222_2cae96477ffd7f603419189e2662dce5.pdf
Cairo University, Faculty of Dentistry
Advanced Dental Journal
2636-302X
2636-3038
3
3
2021
07
01
Infra-zygomatic mini screws compared to high pull headgear for distalization and intrusion of maxillary molars in growing patients with class II malocclusion: a randomized clinical trial
135
150
EN
Ahmed
Afify
Department of Orthodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt.
ahmed.khaled@dentistry.cu.edu.eg
Amr
El Dakroury
Department of Orthodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt.
amr.eldakroury@dentistry.cu.edu.eg
Mostafa
El Dawlatly
Department of Orthodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt.
mostafaeldawlatly@gmail.com
Sherif
Ali
El Kordy
Department of Orthodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt.
sherif.kordi@hotmail.com
10.21608/adjc.2021.55081.1088
Objective: The aim of the present randomized clinical study was to evaluate the distalization and intrusion effect of an Infra-zygomatic mini-screws supported appliance and compare it with high pull headgear appliance in treatment of growing patients with class II malocclusion.<br /> <br /> Methodology: 22 growing boys aged between (10 to 12 years) with class II div 1 malocclusion randomly divided to 2 equal groups. The first group treated with high pull headgear with acrylic splint and the second one treated with an Infra-zygomatic mini-screws supported appliance. The treatment duration was 8 months for both groups. Lateral cephalometric radiographs were taken before and after the treatment for each patient to be analyzed.<br /> <br /> Results: The maxillary first molar distalization was 2.58 ± 2.31 mm in head gear group and 1.53 ± 2.83 mm in mini-screws group. There was no significant maxillary first molar intrusion in both groups. There were no significant differences between the two groups.<br /> <br /> Conclusions: The mini-screws supported appliance can be used as the fixed replacement of the removable high pull headgear appliance with no need for patient cooperation.
Infra-zygomatic,Mini-screws,headgear,Distalization,Class II
https://adjc.journals.ekb.eg/article_185414.html
https://adjc.journals.ekb.eg/article_185414_d41d8cd98f00b204e9800998ecf8427e.pdf
Cairo University, Faculty of Dentistry
Advanced Dental Journal
2636-302X
2636-3038
3
3
2021
07
01
Comparison Study of Microbial Growth of Injection Molded Peek and Bre.Flex Materials for Bounded Removable Partial Dentures: A Randomized Clinical Trial
151
158
EN
Moataz
Ibrahim
Hashim
ministry of health
moataz.hashim@dentistry.cu.edu.eg
10.21608/adjc.2021.23908.1052
<strong>Aim:</strong> to compare the adhesion of bacteria by using Brain heart infusion agar plates in class III modification I maxillary partially edentulous patients wearing removable partial dentures constructed from two different injectable thermoplastic materials PEEK and BRE.FLEX (second edition).<br /> <strong>Materials and methods</strong>: twenty-six patients having Kennedy class III modification I Maxillary partially edentulous ridges with fully dentate mandibular arch were divided into two equal groups to fabricate the thermoplastic materials. The first group received Bre.Flex material. The second group received PEEK material. The adhesion of bacteria from two groups were measured by using (brain heart infusion agar plate): at the time of denture insertion, after one week and after four weeks of denture insertion. The collected data were tabulated and statistically analyzed.<br /> <strong>Results</strong>: Within the Bre.Flex group, the bacterial growth has increased as the follow up period increased. Within the PEEK group, the bacterial count has increased as the follow up period increased. The PEEK group showed a greater bacterial growth than the Bre.Flex group in all the follow up periods and the difference was statistically significant.<br /> <strong>Conclusion</strong>: While both denture base materials have the affinity to support bacterial growth, Bre.flex dentures have the less affinity to support aerobic and anaerobic bacterial growth.
PEEK,thermoplastic materials,bacterial growth
https://adjc.journals.ekb.eg/article_200660.html
https://adjc.journals.ekb.eg/article_200660_c7a320d50a32f900cebefbb5e4e44504.pdf