@article { author = {Aidaros, Nawal and Kamh, Raghda}, title = {Effect of Incorporating Nano-Pearl and Nano-Seashell Particles into Fluoride-based Pits and Fissure Sealant on Enamel Remineralization of Permanent Extracted Molars: An In Vitro Study.}, journal = {Advanced Dental Journal}, volume = {3}, number = {4}, pages = {159-172}, year = {2021}, publisher = {Cairo University, Faculty of Dentistry}, issn = {2636-302X}, eissn = {2636-3038}, doi = {10.21608/adjc.2021.80816.1101}, abstract = {Objective: This in-vitro study evaluated the remineralizing effect of nano-pearl and nano-seashell incorporated into fluoride-based pits-and-fissures sealants by assessing the enamel surface microhardness and the enamel elemental analysis.   Materials and Methods: 75 extracted third molars were randomly allocated to one of the following 5 groups of fluoride containing sealant: 10% nano-pearl; 15% nano-pearl; 10% nano-seashell; 15% nano-seashell; or plain fluoride containing sealant. After 2 weeks of dynamic pH-cycling using Pepsi as demineralizing solution; 10 molars of each group were subjected to microhardness assessment, while 5 molars of each group were subjected to Energy Dispersive X-ray Analysis. Results: Teeth treated with 15% nano-pearl reported the highest percentage increase (20.5%) while teeth treated with plain fluoride containing sealant showed a percentage decrease (-11.62 %). The XRD pattern results confirmed the presence of aragonite and calcite polymorphs of CaCO3 in nano-pearl powder and the presence of aragonite in nano-seashell powder. Calcium ions recorded a significantly higher value in the groups treated with 10% and 15% nano-pearl incorporated pits and fissure sealant. Conclusion: The incorporation of 10 % and 15 % nano-pearl and nano-seashell particles into fluoride-based pits and fissure sealant significantly improved the enamel surface microhardness as well as the enamel mineral content.}, keywords = {seashell,Pearl,remineralization,Pits and fissure sealant,Calcium Carbonate,Enamel Microhardness}, url = {https://adjc.journals.ekb.eg/article_200644.html}, eprint = {https://adjc.journals.ekb.eg/article_200644_e72b5cba7ff9830227093645d07a3eac.pdf} }